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RNA干扰-设计与应用(RNAi-Design and Application)

来源:未知 作者:admin 人气: 发布时间:2009-04-18
摘要:Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi
Part I: Designing Optimal RNAi Tools
1. Principles of Dicer Substrate (D-siRNA) Design and Function
Mohammed Amarzguioui and John J. Rossi . . . . . . . . . . . . . . . . . . . . . . . . 3
2. Expression, Purification, and Analysis of Recombinant
Drosophila Dicer-1 and Dicer-2 Enzymes
Xuecheng Ye and Qinghua Liu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3. In vitro RNA Cleavage Assay for Argonaute-Family Proteins
Keita Miyoshi, Hiroshi Uejima, Tomoko Nagami-Okada,
Haruhiko Siomi, and Mikiko C. Siomi . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
4. Identifying siRNA-Induced Off-Targets by Microarray Analysis
Emily Anderson, Queta Boese, Anastasia Khvorova,
and Jon Karpilow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Part II: Application of RNAi in Diverse Organisms
5. Hydrodynamic Delivery of siRNA in a Mouse Model of Sepsis
Doreen E. Wesche-Soldato, Joanne Lomas-Neira, Mario Perl,
Chun-Shiang Chung, and Alfred Ayala . . . . . . . . . . . . . . . . . . . . . . . . . . 67
6. Nasal Delivery of siRNA
Vira Bitko and Sailen Barik . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
7. RNA Interference as a Genetic Tool in Trypanosomes
Vivian Bellofatto and Jennifer B. Palenchar . . . . . . . . . . . . . . . . . . . . . . . . 83
8. Lentivirus-Mediated RNA Interference in Mammalian Neurons
Scott Q. Harper and Pedro Gonzalez-Alegre . . . . . . . . . . . . . . . . . . . . . . 95
9. Silencing Genes by RNA Interference in the Protozoan Parasite
Entamoeba histolytica
Carlos F. Solis and Nancy Guillén . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
ix
x Contents
10. Use of RNAi in C. elegans
Tsuyoshi Ohkumo, Chikahide Masutani, Toshihiko Eki,
and Fumio Hanaoka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
11. Application of siRNA Against SARS in the Rhesus
Macaque Model
Qingquan Tang, Baojian Li, Martin Woodle, and Patrick Y. Lu . . . . . 139
12. siRNA and shRNA as Anticancer Agents
in a Cervical Cancer Model
Wenyi Gu, Lisa Putral, and Nigel McMillan . . . . . . . . . . . . . . . . . . . . . . . . 159
13. Identification and Expression Analysis of Small RNAs During
Development
Toshiaki Watanabe, Hiroshi Imai, and Naojiro Minami. . . . . . . . . . . . . 173
14. Screening and Identification of Virus-Encoded RNA Silencing
Suppressors
Sumona Karjee, Mohammad Nurul Islam,
and Sunil K. Mukherjee . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 187
15. Application of RNA Interference in Functional Genomics Studies
of a Social Insect
Michael E. Scharf, Xuguo Zhou,
and Margaret A. Schwinghammer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 205
16. Temporal Control of Gene Silencing by in ovo Electroporation
Thomas Baeriswyl, Olivier Mauti, and Esther T. Stoeckli . . . . . . . . . . . 231
17. Altering Flower Color in Transgenic Plants by RNAi-Mediated
Engineering of Flavonoid Biosynthetic Pathway
Yoshikazu Tanaka, Noriko Nakamura, and Junichi Togami . . . . . . . . . 245
18. Transgenic RNA Interference in Mice
Pumin Zhang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 267

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