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HEK293 细胞及其变种

来源:生物病毒网 作者:无城 人气: 发布时间:2010-01-10
摘要:HEK293 (human embryonic kidney293)细胞是实验室常用cell line之一,其变种繁多,有些比293细胞本身应用得更广,如293T。 ATCC number:CRL-1573 描述如下:This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 3

HEK293 (human embryonic kidney293)细胞是实验室常用cell line之一,其变种繁多,有些比293细胞本身应用得更广,如293T。

ATCC number:CRL-1573™
     描述如下:This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2 %. The der(1)t(1;15) (q42;q13), der(19)t(3;19) (q12;q13), der(12)t(8;12) (q22;p13), and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+, and a single Xp+ in most cells.

另外参见:http://www.mbi.ufl.edu/~shaw/293.html,是一个非常全的总结。


293A
QBI-HEK 293A Cells


The QBI-HEK 293A cell line is an immortalized line of primary human embryonic kidney cells transformed by sheared human Adenovirus type 5 (Ad5) DNA. QBI-HEK 293A cells, harboring the E1A and E1B region of the adenoviral genome, complement in trans the deletion of the E1 region in the recombinant Adenovirus. Therefore, the QBI-HEK 293A cell line is an excellent packaging cell line for the generation of high titer recombinant Adenovirus.

In addition, and contrary to other HEK 293 cell lines, Qbiogene's QBI-HEK 293A cell line is a superior sub-clone of HEK 293 cells and strongly adheres to plastic dishes. QBI-HEK 293A cells do not require any special plasticware as long as it is treated for cell culture.

Furthermore, the QBI-HEK 293A cell line is very reliable, performing extremely well in plaque assay and transfection experiments.

QBI-HEK 293A cells are available in all Qbiogene Adenoviral Vector Systems and serve as the standard packaging cell line for our Custom Adenovirus Services. These services are also available with our own RCA free (Replication Competent Adenovirus free) cell line BMAdE1™.

For further information on our RCA free cell line BMAdE1™, please refer to the MERLIN section of this catalog, or contact us at adenovirus@qbiogene.com.

Advantages

Superior sub-clone of HEK 293 cells that adheres strongly to plastic dishes
Reliable post-crisis cell line
Grow easily
Perform especially well in plaque assays and in transfection protocols
Ideal for the generation of helper independent recombinant Adenovirus
Tested for the absence of mycoplasma

来自:http://www.krackeler.com/products/fid/2692

其实说白了,就是包装腺病毒用的。


293T

是目前应用最为广泛的病毒包装细胞系之一。
293T is a derivative of 293 cells that stably express
the large T-antigen of SV40.  Plasmids containing the SV40
origin are replicated to a copy number of between 400 -
1000 plasmids per cell within 293T, and therefore express
at a higher level.

293T are also resistant to G418.  This cell-line was made
in Michelle Calos' lab at Stanford about 14 years ago.
来自:http://www.bio.net/bionet/mm/virology/2002-March/007481.html

说白了就是包装含SV40的vector的。如逆转录病毒,漫病毒。


293FT


就是293T的fast grow的变种。
invitrogen如是说:Description:
The 293FT Cell Line is a clonal isolate derived from transformed embryonal kidney cells. The 293FT strain is a fast-growing highly transfectable variant of the 293 cell line, which contains the large T antigen for high-level expression of the packaging proteins contributing to higher viral titers.
试用过,感觉产病毒不如我的293T好,不知道是不是方法理想还是细胞不好。


Phoenix

也是293T的变种。
Phoenix helper-free retrovirus producer lines:

Phoenix is a second-generation retrovirus producer lines for the generation of helper free ecotropic and amphotropic retroviruses. The lines are based on the 293T cell line (a human embryonic kidney line transformed with adenovirus E1a and carrying a temperature sensitive T antigen co-selected with neomycin).

来自:http://www.stanford.edu/group/nolan/retroviral_systems/phx.html
 

 

作者:无城

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